Differential expression of prostaglandin F receptor in goat endometrium during estrous cycle and early pregnancy

Aim: Present study was undertaken to investigate profiling of prostaglandin F receptor (PGFR) mRNA expression during estrous cycle and early pregnancy. Materials and Methods: Female genitalia of goats (n=3) were collected from a local abattoir. Based on color, vasculature, size, and consistency of corpus luteum (CL), the uteri were classified into three stages of the estrous cycle: stage I: Days 3–5; stage II: Days 6–15, stage III: Days 16–21 and early pregnancy (<30 d foetus). Total RNA was isolated from endometrial scrapings of respective uteri and reverse transcribed followed by DNase treatment. cDNA was used as template to assess gene expression. Expression profiling was performed for PGFR gene using Real Time quantitative PCR (RT-qPCR). Results: At the time of luteolysis the expresion of PGFR was found to be high. However, during pregnancy the expression of PGFR was high as compared to stages of estrous cycle. Conclusion: Higher expression of PGFR at stage III showed luteolytic signal during estrous cycle. However, peaked expression during pregnancy revealed that the conceptus could be responsible for upregulated expression of PGFR in the endometrium during the implantation period.